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khtT [2019-05-08 09:46:48]
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khtT [2019-05-08 09:46:48]

K+/H+ antiporter for K+ efflux
locus
BSU09860
pI
4.89
mw
18.59 kDa
protein length
165 aa Sequence Blast
gene length
498 bp Sequence Blast
function
potassium ion efflux
product
K+/H+ antiporter for K+ efflux
essential
no
synonyms
yhaT
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.5|Metal ion transporter]
  • [category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.1|Metal ion homeostasis (K, Na, Ca, Mg)] → [category|SW 1.3.1.3|Potassium uptake/ export]
  • [category|SW 3|Information processing] → [category|SW 3.5|Targets of second messengers] → [category|SW 3.5.1|Targets of c-di-AMP]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    1,060,427 → 1,060,924

    The protein

    [SW|Domains]

  • contains a [SW|RCK_C domain] at the C-terminus (according to UniProt, [http://www.uniprot.org/uniprot/?query=RCK+C-terminal+domain&sort=score x])

    • [SW|Localization]

  • cell membrane (peripheral) (according to UniProt)

    • Expression and Regulation

    Operons

    genes
    [gene|A54E8E38AEF596D09B4BEB64D9F539A8AD34E73D|khtS]-[gene|D8A8FBC05B9665F72A88685D2B33822C2BB84C62|khtT]-[gene|9A54C82323BA9220CAA6041E3745FAB9E222FE78|khtU]
    description
    [Pubmed|22383849]
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    Biological materials

    Mutant

  • MGNA-A675 (yhaT::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/675 NBRP B. subtilis, Japan]
  • GP2078 (Δ[gene|A54E8E38AEF596D09B4BEB64D9F539A8AD34E73D|khtS]-[gene|D8A8FBC05B9665F72A88685D2B33822C2BB84C62|khtT]-[gene|9A54C82323BA9220CAA6041E3745FAB9E222FE78|khtU]::''aphA3''), available in [SW|Jörg Stülke]'s lab
  • BKE09860 (Δ[gene|D8A8FBC05B9665F72A88685D2B33822C2BB84C62|khtT]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE09860 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATGAAAATCCCTCCGAAT, downstream forward: _UP4_TCTGGAGAGGGCGTGTGAGC
  • BKK09860 (Δ[gene|D8A8FBC05B9665F72A88685D2B33822C2BB84C62|khtT]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK09860 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATGAAAATCCCTCCGAAT, downstream forward: _UP4_TCTGGAGAGGGCGTGTGAGC

    • Expression vectors

  • pGP2906 (N-terminal His-tag, purification from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab

    • References

    Reviews

  • 27935846

    • Original publications

  • 14987767,17679694,24330391